You have the choice of computing the structural alignment using 3 approaches:
- based on the pairwise, global sequence alignment between the selected chains using the well-known least-squares fit (LSQ)
- based on MAMMOTH, a method not requiring any sequence similarity between the two structures, finding the best geometrical fit
- by giving the transformation matrix
Structural alignment based on the pairwise sequence alignment:
- http://i.moltalk.org/strx2strx/6/PDB/1QMZ/65/PDB/1FIN/65/?/seqbased
Structural alignment based on a strictly geometrical fit (MAMMOTH):
- http://i.moltalk.org/strx2strx/6/PDB/1QMZ/65/PDB/1FIN/65/?/coordsbased
parameters:
- toolchain
- "strx2strx"
- position
- "6"
- strx source 1
- "PDB" or "model"
- strx id 1
- first structure, 4-letter pdb code or unique identifier of uploaded model
- chaincode 1
- first chain, ASCII value of chain identifier (A=65, B=66, ' '=32, ...)
- strx source 2
- "PDB" or "model"
- strx id 2
- first structure, 4-letter pdb code or unique identifier of uploaded model
- chaincode 2
- second chain, ASCII value of chain identifier (A=65, B=66, ' '=32, ...)
- transformation matrix
- only for action "trafobased"
- alaction
- either "seqbased" for the sequence alignment based structural alignment or "coordsbased" for the strictly coordinates based structural alignment (MAMMOTH) or "trafobased" for applying the explicitly passed transformation matrix